Dionex HPLC System GP50 Gradient Pump Injector ED40 l Detector

Dionex DX 500 chromatography are analytical state-of-the-art systems for ion chromatography (IC) and liquid chromatography (HPLC) 500 applications

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The system includes: Dionex GP50 gradient pump, injector, ED40 Electrochemical Detector, AS 50 passer with two additional samples racks, DS3 detection stabilizer model DS3-1, Netgear 8 Port 10 Mbps Hub EG40 Validation Cell ED40/CD20 Validation Cell and Accessories


Dionex DX 500 chromatography are analytical state-of-the-art systems for ion chromatography (IC) and liquid chromatography (HPLC) 500 applications.DX modules are characterized by their ease of use, configuration system flexible and adaptable to a variety of analytical needs. The operation can be controlled directly from the front panel of each module, wich or without the creation of methods. The addition of the DX LAN interface and PeakNet Software completely automates the operation of the system

Haue the modules were designed to maximize the number of common characteristics and thus reduce training time for new users. Operating controls and device status is displayed on a liquid crystal (LC) display. The keypad and LCD are the command center from which you access a series of menus and screens that determine the operating parameters of the instrument and provide diagnostic information. Using live at the bottom of each screen briefly describes the attributes of any function of the cursor. A more detailed help is always available by pressing the Help button below the screen.

The modules are housed in rugged housings, corrosion resistant and can be stacked to save work bench or table space. Figure 1-1 an option for setting up a dual-channel system. Note that the pump GP40 gradient or isocratic pump IP20, the

heavy module is always located at the bottom of the stacked system. The LC30 chromatography oven is always installed to the left of the other modules. In a two channel system, placing the second channel to the left of the LC30.

System Description

The air pressure required to pump and valves operate airoperated penetrates tha the rear panel of each module.

Direct connections between the liquid components of the system are made to fittings on the front panels. Eluent of the pump enters the injection valve in the chromatography module. The sample loop is filled through the injection orifice on the side of the injection valve Rheodyne the chromatography module. From there, the flow and eluting the sample through the column (s) to the detection cell.

Liquid waste from the pump and the module output chromatography through the rear walls.

ED40 Electrochemical Detector

The electrochemical detector ED40 measure resulting from the application of the potential (voltalte) between electrodes in flow-cell current. According to a method by which the potential is applied and current measured, several different properties of the solution flow can be determined. These measures will help to answer key questions Analytical Chemists ask: What's in it and how is mud?

Of course, other sensors are used in liquid chromatography (HPLC). The UV-visible absorbance detector dominates all other forms of detection. Sensitivity is excellent for analytes Mang, in particular aromatic species and transparent mobile phases are readily available. However, there are many analytes Haue very poor absorption and are not detected with sufficient sensitivity by UV absorbance. Most of these chromophores are aliphatic molecules and inorganic ions, organic molecules. UV detection at low wavelen-e can be used, but to a loss of selectivity. Detecting refractive index can also be used. However, maintaming a stable base can be difficult, and RI detection is less sensitive and much less selective than UV detection.

Fortunately, a wide variety of chromophores molecules can be detected with a good or excellent sensitivity by one of the many forms of electrochemical detection. These molecules include carboxylic, sulfonic and phosphonic acids, alcohols, glycols, aldehydes, and carbohydrates, primary, secondary, tertiary and quaternary, sulfates, sulfoxides, thiols, sulfides, and mercaptans and anions and cations Morganic. In addition, compared to the UV absorbance detection, electrochemical detection provides substantial improvements in the sensitivity and selectivity for amine and hydroxy-substituted aromatics such as catecholamines.

Several forms of haue electrochemical detection HPLC become popular for some applications. Conductivity is the detection method workhorse in ion chromatography, as the UV detection HPLC. DC amperometry is the preferred method for neurochemical analyzes. Pulsed amperometric is now established as the method of detection than for carbohydrates. For most numerous analytes listed in the previous paragraph, detection by UV-visible absorbance is poor, while one of the three main techniques of electrochemical detection provided by the ED40 provides better sensitivity and selectivity.

Electrochemical detection is not a substitute for UV-visible absorbance detection, but is an important complement. A liquid chromatograph equipped wich both a Dionex AD20 Absorbance Detector and an electrochemical detector ED40 is a powerful and versatile tool for analysis.

Detection modes

The ED40 provides three main types of electrochemical detection: conductivity, DC amperometry and integrated amperometry. Pulsed amperometry is a form of integrated Amperometry.

• detecting the conductivity is based on measuring the intensity of the transported from dissolved ions in an electric field electric current.

• amperometric detection DC is based on the measurement of the oxidation or reduction (electrolysis) of analyte molecules on the surface of an electrode resulting current.

• the integrated detection Aniperonietric pulsed DC amperometry are similar in that the molecules are oxidized or reduced at the surface of an electrode. However, the current is measured by integration Durine, a portion of a potential with respect to the repetition time waveform.

In addition, the method of voltammetry is used to determine the potential used in DC and o Amperometry Interated.

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